Merck
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Y0626

Sigma-Aldrich

Yeast Nitrogen Base Without Amino Acids

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

form

powder

technique(s)

microbe id | utilization test: suitable

pH

5.2-5.6(0.67% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts

General description

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Application

Yeast Nitrogen Base Without Amino Acids has been used:
  • in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization
  • as a component of selective liquid media for uracil auxothrophic growth in yeast
  • to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Preparation Note

1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Yeast transformation efficiency is enhanced by TORC 1-and eisosome-dependent signaling
Yu SC, et al.
MicrobiologyOpen, e00730-e00730 (2018)
Elisa Michelini et al.
Nature protocols, 3(12), 1895-1902 (2009-01-31)
We report a step-by-step protocol describing how to develop and use a yeast-based bioassay for androgen-like compounds. Saccharomyces cerevisiae cells are genetically engineered to express the human androgen receptor (hAR) and the bioluminescent (BL) reporter gene luciferase (from Photinus pyralis)
Patrick F Suthers et al.
Metabolic engineering communications, 11, e00148-e00148 (2020-11-03)
Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial production of organic acids by microorganisms.
Peter Hayek et al.
Microbiological research, 165(3), 250-258 (2009-07-21)
Various factors are thought to be responsible for Candida albicans virulence, such as lipases, proteases and adhesins. Many of these factors are GPI-anchored cell surface proteins responsible for pathogenicity. Hwp2 is a putative GPI-anchored protein. The purpose of this study
Activating and elucidating metabolism of complex sugars in Yarrowia lipolytica
Ryu S, et al.
Applied and Environmental Microbiology, 82(4), 1334-1345 (2016)

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