Merck
All Photos(3)

C2605

Sigma-Aldrich

Cellulase from Aspergillus sp.

greener alternative

aqueous solution

Synonym(s):
Carezyme, Carezyme 4500L
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

Quality Level

form

solid

specific activity

≥1000 units/g

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Cellulase from Aspergillus sp. has been used to degrade cello-oligosaccharides into glucose during the analysis of BBC (bioabsorbable bacterial cellulose) biodegradability. The enzyme has also been used in photon correlation spectroscopy to study its effects on unfiltered beer samples. The parameters evaluated in this spectroscopic analysis were the size of suspended particles, flow rate and haze stability.

Packaging

50, 250 mL in glass bottle

Biochem/physiol Actions

Cellulases are enzymes that hydrolyze cellulose, a linear polymer of anhydroglucose units linked together by β-1,4-glycosidic bonds, to glucose. Endo-β-D-glucanase is one of the major component enzymes of the cellulase complex. It catalyzes the hydrolysis of cellulose by randomly splitting the sugar residues within the molecule. Exo-β-D-glucanase and β-glucosidase can synergistically convert cellulose into glucose and hence are used on an industrial scale.

Preparation Note

produced by submerged fermentation of a genetically modified Aspergillus microorganism

Legal Information

A product of Novozyme Corp.
Carezyme is a registered trademark of Novozymes Corp.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the density of Product C2605, Cellulase from Aspergillus sp.?

    The density of this cellulase solution is approximately 1.2 g/mL.

  4. How are the activity units of Product C2605, Cellulase from Aspergillus sp., listed on the C of A?

    The activity of cellulase given on the C of A is in units per gram of liquid.

  5. What are the supplier's working conditions for determining the activity for Product C2605, Cellulase from Aspergillus sp.?

    This product is assayed using carboxymethycellulose as substrate, at 40°C, at a pH of 7.5.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the unit activity of the Cellulase from Aspergillus sp. enzyme, Product C2605?

    We provide the unit activity of the product in terms of units/g, for each individual lot. To get a units/mL value, one can use the density value of approximately 1.2 g/mL to convert to a units/mL value for each lot.

  8. What is the optimum pH for Cellulase from Aspergillus sp., Product C2605?

    At a temperature of 40°C, Novozyme has found ~pH 8.0-8.2 to be the optimum pH range for this enzyme.

  9. What is the optimum temperature for activity for Cellulase from Aspergillus sp., Product C2605?

    At pH 8.5, Novozyme has determined that the optimal activity for this enzyme is ~60°C. The percentage of maximum activity is ~40% of that value at 40°C, and ~80% at 50°C. There is a steep drop in activity above 60°C.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Monitoring of Beer Filtration Using Photon Correlation Spectroscopy (PCS).
Sensidoni M, et al.
Journal of the Institute of Brewing, 117(4), 639-646 (2011)
Pierre-Louis Bombeck et al.
Biotechnology for biofuels, 10, 293-293 (2017-12-12)
Lignocellulosic biomass will progressively become the main source of carbon for a number of products as the Earth's oil reservoirs disappear. Technology for conversion of wood fiber into bioproducts (wood biorefining) continues to flourish, and access to reliable methods for
Muhammad Sohail et al.
New biotechnology, 25(6), 437-441 (2009-06-26)
Fungal cellulases are well-studied enzymes and are used in various industrial processes. Much of the knowledge of enzymatic depolymerization of cellulosic material has come from Trichoderma cellulase system. Species of Trichoderma can produce substantial amounts of endoglucanase and exoglucanase but
Yang Hu et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 97(1), 114-123 (2011-02-04)
Cellulose biodegradation resulting from enzymolysis generally occurs in nature rather than in the human body because of the absence of cellulose degrading enzymes. In order to achieve in-vivo degradation in human body for in-vivo tissue regeneration applications, we developed a
Katherine J Mackenzie et al.
Journal of the American Chemical Society, 135(1), 293-300 (2012-12-29)
Here we report the construction and characterization of a recoverable, thermoresponsive polymer-endoglucanase bioconjugate that matches the activity of unmodified enzymes on insoluble cellulose substrates. Two copolymers exhibiting a thermoresponsive lower critical solution temperature (LCST) were created through the copolymerization of

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