Merck
All Photos(6)

C8546

Sigma-Aldrich

Cellulase from Trichoderma reesei ATCC 26921

greener alternative

lyophilized powder, ≥1 unit/mg solid

Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

form

lyophilized powder

Quality Level

specific activity

≥1 unit/mg solid

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

solubility

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

greener alternative category

Enabling

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose; to study the biodegradability of bioabsorbable bacterial cellulose (BBC) material.
Cellulase from Trichoderma reesei has been used in a study to assess the differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE. Cellulase from Trichoderma reesei has also been used in a study to investigate the liquefaction of hydrothermally pretreated wheat straw at high-solids content.

Packaging

2500, 5000, 10000 units in glass bottle

Biochem/physiol Actions

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. It is also applied in the biodegradation of paper products such as foolscap paper, filter paper, newspaper and office paper as well as microcrystalline cellulose.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15% polyhexamethylene biguanide (PHMB) at 5 mg/mL concentration.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

J P H van Wyk et al.
Bioresource technology, 86(1), 21-23 (2002-11-08)
Environmental issues such as the depletion of non-renewable energy resources and pollution are topical. The extent of solid waste production is of global concern and development of its bioenergy potential can combine issues such as pollution control and bioproduct development
Thomas Portnoy et al.
Eukaryotic cell, 10(2), 262-271 (2010-12-21)
Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly being investigated for second-generation biofuel production from lignocellulosic biomass. The induction mechanisms of T. reesei cellulases have been described recently, but the regulation of the genes
Nóra Szijártó et al.
Bioresource technology, 102(2), 1968-1974 (2010-10-05)
Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A
Evgenij Lyagin et al.
Biotechnology journal, 5(8), 813-821 (2010-07-30)
The screening of catalysts, substrates or conditions in the early stages of bioprocess development requires an enormous number of experiments and is a tedious, expensive and time-consuming task. Currently available screening systems can only be operated in batch or fed-batch
Laura J Grenville-Briggs et al.
Fungal biology, 117(3), 163-172 (2013-03-30)
The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrumsmall tyrosine rich (PoStr)) proteins.

Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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