DPBS and other basal salt solutions are multi-component formulations. In total, this product requires 9.6 g to prepare 1L of a 1X solution. A molar concentration will not apply to the entire final product, only to the concentration of the individual components. See the link below for a formulation table for the DPBS products. The concentration of each component in these formulations are listed:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/354/091/d5773for.pdf
D5773
Dulbecco′s Phosphate Buffered Saline
Without calcium chloride, powder, suitable for cell culture
Synonym(s):
DPBS
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This Item | 59300C | D8662 | D4031 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C |
DPBS, Isotonic saline, cell washing solution, cell suspension solution | DPBS, Isotonic saline, cell washing solution, cell suspension solution | DPBS, Isotonic saline, cell washing solution, cell suspension solution | DPBS, Isotonic saline, cell washing solution, cell suspension solution |
Application
Use this formulation of DPBS when tissue or cell dissociation or release is the objective. Also, suitable for studies that involve calcium metabolism.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
used together
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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Hello, it is my first time buying DPBS powder. May I know how much should I weigh to prepare 1M of solution? My product is D5773-10L. Thank you.
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