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D6046

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
DME, DMEM
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: yes
phenol red: yes
glucose: low

shipped in

ambient

storage temp.

2-8°C

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General description

This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose is a fully supplemented liquid form of the original Dulbecco′s formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
Dulbecco′s Modified Eagle′s Medium - low glucose has been used in the maintenance/culturing of mammalian cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Huong Thi Pham et al.
PLoS genetics, 14(8), e1007574-e1007574 (2018-08-04)
The broadly conserved bacterial signalling molecule cyclic-di-adenosine monophosphate (c-di-AMP) controls osmoresistance via its regulation of potassium (K+) and compatible solute uptake. High levels of c-di-AMP resulting from inactivation of c-di-AMP phosphodiesterase activity leads to poor growth of bacteria under high...
Nicotine increases cancer stem cell population in MCF-7 cells.
Hirata N et al.
Biochemical and Biophysical Research Communications, 403, 138-138 (2010)
Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose.
Anthonisen EH et al.
The Journal of Biological Chemistry, 285, 1607-1607 (2010)
Takaho Tsuchiya et al.
PLoS computational biology, 13(12), e1005913-e1005913 (2017-12-28)
Cells decode information of signaling activation at a scale of tens of minutes by downstream gene expression with a scale of hours to days, leading to cell fate decisions such as cell differentiation. However, no system identification method with such...
June Guo et al.
Metabolism: clinical and experimental, 68, 108-118 (2017-02-12)
Our laboratory has shown that insulin's effect to decrease neointimal thickness after arterial injury is greatly diminished in insulin resistant conditions. Thus, in these conditions, a better alternative to insulin could be to use an insulin sensitizing agent. Metformin, the...

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