D9779

DL-Dithiothreitol

for molecular biology, ≥98% (HPLC), ≥99% (titration)

• Synonym(s): threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• EC Number: 222-468-7
• MDL number: MFCD00004877
• PubChem Substance ID: 24894330
• NACRES: NA.31

Properties

• grade: for molecular biology
• Quality Level: 200
• assay: ≥98% (HPLC); ≥99% (titration)
• form: powder
• reaction suitability: reagent type: reductant
• mp: 41-44 °C (lit.)
• solubility: H₂O: 50 mg/mL
• cation traces: heavy metals (as Pb): ≤5 ppm
• foreign activity: DNase, RNase, protease, none detected
• storage temp.: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

General description

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Application

DL-Dithiothreitol has been added to the samples before loading them for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used in the procedure of in situ hybridization of mRNA.

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

250 mg in poly bottle

1, 5, 5, 10, 25, 50 g in poly bottle

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318
• Precautionary Statements: P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves