Sigma′s GenElute™ FFPE DNA Purification Kit provides a rapid method for the isolation and purification of genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Sigma′s GenElute™ FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The DNA is purified from other cellular components without the use of phenol or chloroform. The purified genomic DNA is of the highest integrity, and can be used in a number of downstream applications including qPCR, mutation screening, microarray analyses, sequencing, Southern blotting and SNP analysis.
Fast and easy processing using rapid spin-column format
High yields of gDNA
No phenol or chloroform extractions
PCR and Quantitative PCR
Features and Benefits
High quality and integrity of the isolated DNA - The purified DNA is of the highest quality and integrity, and can be used in any sensitive downstream applications.
High yields - Norgen′s FFPE DNA Purification Kit allows for the purification of high yields of DNA.
No phenol:chloroform extractions - DNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
Rapid procedure - Isolate DNA from FFPE tissue sections using a rapid spin column format in as little as 1 hour
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