Merck
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F7524

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):
FCS, serum, FBS, sera, Fetal Calf serum, Fetal Calf sera
MDL number:
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

not available in USA, Canada

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

FBS is used in a broad range of cell culture applications.

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth promoting and survival enhancing factor to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Features and Benefits

F7524 is the same great product as Biochrom GmbH. product S0115.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Is product F7524 heat inactivated?

    Not all Fetal Bovine Serum products are heat inactivated. Product F9665 is the heat-inactivated version of F7524.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Is Product F7524, Fetal Bovine Serum, available in the US or Canada?

    No, Product No. F7524 is only available in Europe.

  5. Where is Product F7524, Fetal Bovine Serum, sourced from?

    Product is South American Origin and EU approved.  Some batches could be sourced from France.

  6. Is Product F7524, Fetal Bovine Serum, a USDA approved serum?

    This is a non-USDA approved serum. The serum is tested for BVD (bovine virus diarrhea), PI3 (parainfluenza type 3), and IBR (infectious bovine rhinotracheitis) only.

  7. How is Product F7524, Fetal Bovine Serum, sterilized?

    This product is sterilized by filtration through three (3) sterile filter membranes, each of which has a pore size of 0.1 microns.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Does Sigma-Aldrich offer delipidated bovine serum?

    At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

  11. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Articles

Choosing the optimal cell culture media for vaccine production

Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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