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G1019

Sigma-Aldrich

Gamborg′s Vitamin Solution

1000 ×, liquid, suitable for plant cell culture

NACRES:
NA.72

sterility

sterile-filtered

Quality Level

form

liquid

concentration

1000 ×

application(s)

cell culture | plant: suitable

color

colorless

pH

5.0-7.0

Featured Industry

Agriculture

storage temp.

2-8°C

Application

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.

Components

Solution contains (mg/ml): 100.0 myo-inositol, 1.0 nicotinic acid, 1.0 pyridoxine hydrochloride, 10.0 thiamine hydrochloride.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

A N Sakamoto et al.
Plant signaling & behavior, 13(7), e1483673-e1483673 (2018-06-27)
Unrepaired DNA damage hinders the maintenance of genome integrity because it blocks the catalytic activity of replicase. The stalled replication fork can be processed through either translesion synthesis (TLS) with specific polymerases, or replication using the undamaged template. To investigate...
Peng Zhao et al.
Developmental cell, 49(6), 882-893 (2019-05-14)
The maternal-to-zygotic transition (MZT) is an essential developmental turning point in both plants and animals. In plants, the timing of MZT and parental contributions to the zygotic transcriptome remain unclear. Here, by overcoming technical limitations, we characterize the Arabidopsis egg...
R Mallon et al.
Cryo letters, 31(1), 24-28 (2010-03-24)
The source of germplasm as well as the technique used for storage of mosses can enhance survival after cryopreservation. Samples of gametophores, protonemata and protonemal brood cells from in vitro cultures of Splachnum ampullaceum were cryopreserved following exposure to a...
Li-Da Wang et al.
Journal of Asian natural products research, 4(3), 171-174 (2002-07-18)
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of...
Nutrient requirements of suspension cultures of soybean root cells.
O L Gamborg et al.
Experimental cell research, 50(1), 151-158 (1968-04-01)

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