Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.
Solution contains (mg/ml): 100.0 myo-inositol, 1.0 nicotinic acid, 1.0 pyridoxine hydrochloride, 10.0 thiamine hydrochloride.