G5003

α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

• Synonym(s): α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
• CAS Number: 9001-42-7
• Enzyme Commission number: 3.2.1.20 (BRENDA, IUBMB)
• EC Number: 232-604-7
• MDL number: MFCD00081321
• NACRES: NA.54

Properties

• type: Type I
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
• contains: phosphate buffer salts and EDTA as balance
• composition: Protein, ≥50%
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• UniProt accession no.: P38158; P53341
• foreign activity: β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
• greener alternative category: Enabling
• relevant disease(s): diabetes (management of non-insulin-dependent diabetes mellitus)
• storage temp.: −20°C
• Gene Information: bakers yeast ... MAL12(853209) , MAL32(852602)

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Application

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Packaging

25, 100 units in glass bottle

1000 units in poly bottle

Sold on basis of p-nitrophenyl α-D-glucoside units.

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Unit Definition

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Analysis Note

Protein determined by biuret.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves