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G9422

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

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Synonym(s):
BGP, Glycerol 2-phosphate disodium salt hydrate
Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Assay

≥99% (titration)

form

powder

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

impurities

≤0.1% Insoluble matter
≤0.5 mol % L-α-isomer

mp

102-104 °C (lit.)

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.001%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.001%
K: ≤0.005%
Mg: ≤0.0005%
Pb: ≤0.001%
Zn: ≤0.0005%

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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This Item
G5422S5136N4645
β-Glycerophosphate disodium salt hydrate BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

Sigma-Aldrich

G9422

β-Glycerophosphate disodium salt hydrate

Premium Grade
Sodium phosphate dibasic BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

Sigma-Aldrich

S5136

Sodium phosphate dibasic

Essential+ Grade
assay

≥99% (titration)

assay

-

assay

≥99.0%

assay

≥97%

form

powder

form

powder

form

powder or crystals

form

powder

technique(s)

cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

≤0.1% Insoluble matter, ≤0.5 mol % L-α-isomer

impurities

≤0.1% Inorganic phosphorus, ≤1.0 mol % L-α-isomer

impurities

-

impurities

-

mp

102-104 °C (lit.)

mp

102-104 °C (lit.)

mp

-

mp

-

General description

Glycerophosphoric acid disodium salt hydrate, also known as BGP, is a versatile compound with applications across various research domains, including cell biology, biochemical research, and metabolomics. Recognized as an endogenous metabolite and a serine-threonine phosphatase inhibitor, BGP is extensively utilized in chitosan hydrogel formulations for drug delivery research. It serves as a phosphate source for cell growth and recombinant protein production, offering advantages in media formulations by reducing precipitation at higher pH levels. Additionally, BGP finds applications in micro-arc oxidation electrolyte solutions, contributing to the creation of photocatalytic coatings.
In cell biology, β-Glycerophosphate disodium salt plays a pivotal role as a phosphate group donor in matrix mineralization studies and accelerates calcification in vascular smooth muscle cells. It also promotes bone matrix mineralization when delivered to osteoblasts, providing a crucial source of phosphate ions. In the realm of biochemical research, BGP functions as a classical serine-threonine phosphatase inhibitor in kinase reaction buffers, offering broad-spectrum inhibition. Its role as an organic phosphate donor is particularly valuable in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. Moreover, BGP is utilized to buffer M17 media for Lactococcus culture in recombinant protein expression. The comprehensive utility of BGP spans multiple areas of research, making it an essential component in studies encompassing cell biology, biochemical research, and metabolomics.

Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Cell culture and Plant cell culture
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • BioUltra Grade for your Cell Biology and Biochemical research

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

comparable product

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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