GUSS

β-Glucuronidase Reporter Gene Staining Kit

Name: β-Glucuronidase Reporter Gene Staining Kit
Brand: SIGMA
Price: 2854 BRL

Synonym(s):

β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase

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1 KIT

R$2,854.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12161503

Key Documents

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usage

kit sufficient for 100 histochemical assays

technique(s)

microbe id | staining: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item	LUC1	G5545	G5420
Sigma-Aldrich GUSS β-Glucuronidase Reporter Gene Staining Kit	Sigma-Aldrich LUC1 Luciferase Reporter Gene Detection Kit	Sigma-Aldrich G5545 Anti-β-Glucuronidase (C-Terminal) antibody produced in rabbit	Sigma-Aldrich G5420 Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) microbe id \| staining: suitable	technique(s) -	technique(s) western blot: 1-2 μg/mL using purified GUS from E. coli	technique(s) western blot: 0.5-1 μg/mL using GUS purified from E. coli
usage kit sufficient for 100 histochemical assays	usage kit sufficient for 100 assays	usage -	usage -
shipped in dry ice	shipped in -	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −70°C	storage temp. −20°C	storage temp. −20°C

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Features and Benefits

Histochemical staining of plant tissues expressing the E. coli GUS enzyme
Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
GUS does not interfere with plant cell function or viability

General description

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Other Notes

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

hcodes

H412

pcodes

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

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Certificates of Analysis (COA)

Lot/Batch Number

0000399755

0000218896

0000121906

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Unexpected consequences of a sudden and massive transposon amplification on rice gene expression.

Ken Naito et al.

Nature, 461(7267), 1130-1134 (2009-10-23)

High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such

Tumor-targeting prodrug-activating bacteria for cancer therapy.

C-M Cheng et al.

Cancer gene therapy, 15(6), 393-401 (2008-03-29)

Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were

Identification of microRNA 395a in 24-epibrassinolide-regulated root growth of Arabidopsis thaliana using microRNA arrays.

Li-Ling Lin et al.

International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)

Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs

Combination cancer therapy by hapten-targeted prodrug-activating enzymes and cytokines.

Kuo-Hsiang Chuang et al.

Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)

Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer

The GUS reporter gene system.

R A Jefferson

Nature, 342(6251), 837-838 (1989-12-14)

The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.

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