Merck
  • L2542
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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

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Synonym(s):
Luria Broth Miller
NACRES:
NA.85

grade

for molecular biology

Quality Level

400

sterility

sterile; 0.2 μm filtered

form

liquid

pH

6.5-7.2

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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L35221.10285L3152
LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

LB broth (MILLER) for microbiology

Millipore

1.10285

LB broth (MILLER)

Supelco

Supelco

L3152

LB broth, Miller

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

-

sterility

-

form

liquid

form

powder

form

medium granules (dehydrated (DCM))

form

-

pH

6.5-7.2

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2 (25 °C in H2O, after autoclaving)

pH

-

storage temp.

room temp

storage temp.

room temp

storage temp.

15-25°C

storage temp.

-

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

Escherichia coli

suitability

-

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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