The volume of this product will vary from lot to lot. The minimum concentration is 5 mg/mL, however a survey of recent lots shows an average of 13 mg/mL. A 1mg vial will have an average volume of 77 uL. The exact protein concentration is reported on the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9420#product-documentation
L9420
Luciferase from Photinus pyralis (firefly)
recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Synonym(s):
Luciferase firefly
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
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Product Name
Luciferase from Photinus pyralis (firefly), recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥10×1010 units/mg protein
mol wt
62 kDa
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | L0159 | SRE0045 | SCT151 |
|---|---|---|---|
| specific activity ≥10×1010 units/mg protein | specific activity - | specific activity ≥10×1010 units/mg protein | specific activity - |
| assay ≥98% (SDS-PAGE) | assay - | assay - | assay - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form - |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli | recombinant - |
| mol wt 62 kDa | mol wt - | mol wt 62 kDa | mol wt - |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in ambient |
Application
Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.[1]
General description
Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light.[2] The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.[3]
Other Notes
One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Physical form
Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral
target_organs
Kidney
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
231.8 °F
flash_point_c
111 °C
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Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Tanushree Dangi et al.
Cell reports, 42(3), 112167-112167 (2023-03-02)
mRNA vaccines are effective in preventing severe COVID-19, but breakthrough infections, emerging variants, and waning immunity warrant the use of boosters. Although mRNA boosters are being implemented, the extent to which pre-existing immunity influences the efficacy of boosters remains unclear.
Isabella Nymann Westensee et al.
Small (Weinheim an der Bergstrasse, Germany), 17(24), e2007959-e2007959 (2021-05-11)
Artificial cells (ACs) aim to mimic selected structural and functional features of mammalian cells. In this context, energy generation is an important challenge to be addressed when self-sustained systems are desired. Here, mitochondria isolated from HepG2 cells are employed as
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Articles
seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
Bioluminescence imaging (BLI) systems allows for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.
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