The LookOut Mycoplasma Elimination Kit is designed to efficiently eliminate mycoplasma contamination from cell cultures. This kit uses a combination of biological agents to reliably and completely remove mycoplasma contamination. The initial treatment is generally sufficient for mycoplasma elimination in most applications. A second step involves a follow-up antibiotic treatment to suppress and inactivate any remaining mycoplasma. The reagents have minimal cytotoxic properties, and the development of resistant strains is highly unlikely due to the significantly reduced mycoplasma concentration after the initial treatment. Compared to other products for mycoplasma elimination, inactivation, or suppression, the LookOut Mycoplasma Elimination Kit is more effective and has not been shown to cause changes in normal cell characteristics. It is the least cytotoxic method, with overall cytotoxicity demonstrated to be less than 20% across a diverse group of cell lines
MP0030
LookOut® Mycoplasma Elimination Kit
Synonym(s):
Mycoplasma contamination treatment
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R$2,289.00
R$2,289.00
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About This Item
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usage
kit sufficient for 5 mL mycoplasma elimination
Quality Level
packaging
pkg of 1 kit
storage condition
protect from light
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
1 of 4
This Item | MP0040A | QR0100 | MP0035 |
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) RT-qPCR: suitable | technique(s) PCR: suitable |
| usage kit sufficient for 5 mL mycoplasma elimination | usage sufficient for 25 reactions | usage sufficient for 250 reactions | usage - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging pkg of 1 kit |
| storage condition protect from light | storage condition dry at room temperature | storage condition - | storage condition - |
mycoplamsa removal agent, MRA, | mycoplasma detection, qPCR-based assay | - | mycoplasma detection, PCR-based assay |
| application(s) detection | application(s) detection | application(s) - | application(s) detection |
General description
Application
Features and Benefits
- Minimal cytotoxic properties of the reagents
- Highly effective
- Retention of normal cell characteristics
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Disclaimer
related product
Storage Class Code
10 - Combustible liquids
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Articles
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Overview of good cell banking practices for cell line cryopreservation purposes.
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.
Protocols
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
Related Content
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Has this been tested in primary cell lines (i.e. skeletal muscle) is there any interference with the normal cell physiology?
1 answer-
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What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?
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The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.
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How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?
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The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.
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