PKH67GL

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

• Synonym(s): Green PKH membrane labeling kit
• NACRES: NA.32

Properties

• Quality Level: 200
• packaging: pkg of 1 kit
• storage condition: protect from light
• fluorescence: λ_ex 490 nm; λ_em 502 nm (PKH67 dye)
• detection method: fluorometric
• shipped in: ambient
• storage temp.: room temp

Description

Application

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:

• To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.
• To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.
• In fluorescence imaging.

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Linkage

For additional technical details on PKH and CellVue^® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

• Diluent C 6 x 10
• PKH67 Cell Linker in ethanol .5 mL

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225 - H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 57.2 °F - closed cup
• Flash Point(C): 14 °C - closed cup