Merck
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PP0540

Sigma-Aldrich

GlycoProfile β-Elimination Kit

Sufficient for 24 samples

storage temp.

room temp

Application

The GlycoProfile β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.

Quantity

The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Sodium hydroxide solution, 5.0 M 60 μL

  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

Kit Components Also Available Separately

Product No.
Description
SDS

  • Centrifugal Filter Unit

Pictograms

FlameCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

82.0 °F - closed cup

Flash Point(C)

27.79 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)

Articles

GlycoProfile™ ß-Elimination Kit

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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