R1755

Rhodamine B isothiocyanate

BioReagent, mixed isomers, suitable for protein labeling

• Synonym(s): RBITC
• Empirical Formula (Hill Notation): C₂₉H₃₀ClN₃O₃S
• CAS Number: 36877-69-7
• Molecular Weight: 536.08
• EC Number: 253-248-9
• MDL number: MFCD00136007
• PubChem Substance ID: 329824007
• NACRES: NA.32

Properties

• description: labeling efficiency with bovine albumin > 70%
• Quality Level: 200
• product line: BioReagent
• fluorescence: λ_ex 543 nm; λ_em 580 nm in methanol
• suitability: suitable for protein labeling
• storage temp.: −20°C
• SMILES string: [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
• InChI: 1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
• InChI key: YVSWPCCVTYEEHG-UHFFFAOYSA-N

Description

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:

• Rhodamine isothiocyanate labeling
• Rhodamine-labeled synthetic APF
• Preparations of protein and fluorescent conjugates
• Fluorescent red dye

Packaging

50, 100, 500 mg in glass insert

1 g in glass bottle

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332 - H315 - H319 - H334 - H335
• Precautionary Statements: P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves