Merck
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R4533

Sigma-Aldrich

RNAzol® RT

For processing total and small RNA from human, animal, plant, bacterial,and viral samples

Synonym(s):
total RNA purification, single step RNA purification, single step RNA isolation, total RNA isolation
NACRES:
NA.52

Quality Level

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

General description

RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.

This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.

Application

Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.

Features and Benefits

  • Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
  • High yields and increased quality of isolated RNA
  • Completed at room temperature in less than an hour starting with fresh tissue or cells

Legal Information

RNAZol is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

230.0 °F

Flash Point(C)

110 °C

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

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