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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

Synonym(s):
Super Optimal broth
NACRES:
NA.85

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
M14281.0547085438
Sigma-Aldrich

Sigma-Aldrich

S1797

SOC Medium

MIO Medium suitable for microbiology, NutriSelect® Plus

Millipore

M1428

MIO Medium

SIM medium suitable for microbiology

Millipore

1.05470

SIM medium

Millipore

Millipore

85438

SIM Medium

sterility

0.2 μm filtered

sterility

non-sterile

sterility

-

sterility

non-sterile

form

liquid

form

powder

form

medium granules (dehydrated (DCM))

form

powder

application(s)

agriculture

application(s)

clinical testing
environmental
food and beverages
veterinary, microbiology

application(s)

food and beverages, microbiology

application(s)

clinical testing
environmental
food and beverages
pathogen testing, microbiology

storage temp.

2-8°C

storage temp.

-

storage temp.

15-25°C

storage temp.

-

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

Escherichia coli, nonselective and differential for Enterobacter spp., nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for enterobacteriaceae

suitability

Escherichia coli, coliforms, enterobacteriaceae

suitability

Providencia spp., nonselective and differential for Citrobacter spp., nonselective and differential for Enterococcus spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for Salmonella spp., nonselective and differential for Shigella spp., nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

Quality Level

500

Quality Level

200

Quality Level

200

Quality Level

100

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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