SHC004

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA

shRNA sequence targeting tGFP

• Synonym(s): MISSION^® Control Vectors
• MDL number: MFCD07785395
• UNSPSC Code: 41106609
• NACRES: NA.51

Properties

• product line: MISSION^®
• concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP.[1] The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.[2][3] The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION^® pLKO.1-puro TurboGFP^™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.[4][5][6]

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable