STRN250

Spectrum^™ Plant Total RNA Kit

sufficient for 250 purifications

• Synonym(s): Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
• NACRES: NA.52

Properties

• Quality Level: 200
• usage: sufficient for 250 purifications
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• greener alternative category: Aligned
• storage temp.: 15-25°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

Application

Spectrum^®Plant Total RNA Kit has been used:

• to extract RNA from the material ground in liquid nitrogen
• to isolate total RNA from mutant and wt (wild type) plantlets
• to extract total RNA from leaf samples
• to extract RNA from all plant tissues

Features and Benefits

• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.

Spectrum is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3