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T3449

Sigma-Aldrich

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Synonym(s):

Trypsin

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R$324.00
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100 ML
R$324.00
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About This Item

UNSPSC Code:
12352204
NACRES:
NA.75

R$324.00

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Request a Bulk Order

recombinant

expressed in corn

sterility

sterile-filtered

form

aqueous solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

shipped in

dry ice

storage temp.

−20°C

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This Item
T2601C5789C5914
TrypZean® Solution, 1× recombinant, expressed in corn, sterile-filtered

T3449

TrypZean® Solution, 1×

StableCell™ Trypsin Solution 1X, sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

T2601

StableCell™ Trypsin Solution

Cell Dissociation Solution Non-enzymatic 1x Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

C5789

Cell Dissociation Solution Non-enzymatic 1x

Cell Dissociation Solution Non-enzymatic 1x Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

C5914

Cell Dissociation Solution Non-enzymatic 1x

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

concentration

1 ×

concentration

1X

concentration

1 ×

concentration

1 ×

form

aqueous solution

form

-

form

solution

form

liquid

recombinant

expressed in corn

recombinant

-

recombinant

-

recombinant

-

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

General description

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction[1]
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells[2]
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium[3]

Features and Benefits

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL8259
RNBL4895
RNBL3434
RNBK7718
RNBK4189

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