T4174
Trypsin-EDTA solution
10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride
Synonym(s):
Cocoonase, Tryptar, Tryptase
About This Item
Recommended Products
biological source
Porcine
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-7.6
shipped in
dry ice
storage temp.
−20°C
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Application
Trypsin-EDTA solution is used for the following applications:
- Used as a supplement in cell culture for their maintenance
- In harvesting cells grown to confluence
- to detach lentivirus-transduced macrophages
Biochem/physiol Actions
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Components
Caution
Preparation Note
also commonly purchased with this product
recommended
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
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