Merck
All Photos(1)

53939-U

Supelco

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

Agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤100 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

silica phase

particle size

2.7 μm

pore size

90 Å

surface area

135 m2/g

operating pH

1-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

Isolation and LC-MS Characterization of Illicit Bath Salts in Urine

The combination of ion-exchange SPE with the HILIC HPLC separation provides a novel approach for the testing of problematic bath salt compounds.

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Ascentis ® Express HILIC U/HPLC Columns

For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.

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Protocols

HPLC Analysis of Paraquat and Diquat on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of Paraquat and Diquat on Ascentis® Express HILIC (UV Detection)

Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques

We offer the tools for the analysis of the metabolites; including certified reference standards, enzymes, substrates, and chromatographic products.

Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques

We offer the tools for the analysis of the metabolites; including certified reference standards, enzymes, substrates, and chromatographic products.

HPLC Analysis of Heroin, Cocaine, Amphetamine and MDMA on Ascentis® Express HILIC

Separation of Heroin, analytical standard, ≥98% (HPLC); Cocaine hydrochloride, analytical standard; D-Amphetamine hemisulfate salt solution, 1.0 mg/mL in methanol, analytical standard, for drug analysis; (±)-3,4-Methylenedioxymethamphetamine hydrochloride solution, analytical standard, for drug analysis, 1.0 mg/mL in methanol

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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