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Supel Tox AflaZea

volume 6 mL, pk of 30

Supel Tox


polypropylene tube

Quality Level

product line



bed wt., 1.2 g


pk of 30


solid phase extraction (SPE): suitable


6 mL

matrix active group

Proprietary (Mycotoxins) phase


food and beverages

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General description

The need for a quick, simplistic sample cleanup approach prior to chromatographic mycotoxin analysis has brought about SPE cartridges that significantly decrease sample prep time, increase reproducibility, and are more user friendly as compared to the industry standard immunoaffinity columns. In addition the Supel Tox SPE approach requires less equipment and fewer consumables, providing an additional cost savings .

Unlike the multiple step “bind and elute” strategy implemented when using immunoaffinity columns, the Supel Tox AflaZea, DON, and Tricho SPE cartridges employ an “interference removal” strategy which saves time by eliminating wash steps prior to elution of aflatoxin, zearalenone, deoxynivalenol, and tricothecenes (type A and B), respectively. Cartridges removing interferences associated with analysis of the above and fumonisins (B1 and B2) as well as ochratoxin A are also available as a part of our Supel Tox product offering.

Features and Benefits

  • Removes interferences associated with mycotoxin analysis.
  • Better reproducibility than the current industry standard, immunoaffinity columns.
  • Time associated with sample preparation is up to ten times faster than that related to immunoaffinity columns.
  • Basic and quick methodology requiring little additional method development.
  • Improved shelf life over immunoaffinity columns due to thermally stable format. No refrigeration required for shipping and storage.

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Do the Supel Tox AflaZea, Don, Tricho, and OchraBind tubes need to be conditioned/equilibrated?

    It's not necessary to condition/equilibrate Supel Tox AflaZea, Don, Tricho and OchraBind tubes.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.


A Comparison of Solid Phase Extraction (SPE) Products Used to Purify Peanut Paste Extract for Aflatoxin Analysis

Supel Tox AflaZea SPE produced exceptional analyte recoveries with good reproducibility for all aflatoxins while requiring less than half the sample volume required by competing products.HPLC analysis of all spiked samples after SPE cleanup showed that all compared SPE cartridges effectively eliminate interferences.

Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis

Analyte recovery values from spiked cannabis extracts ranged from 102 to 127% with RSD below 12% for 3 replicates. Excellent recovery values were even observed for Aflatoxins B2 and G2 which were spiked at a low level of 6.1 ppb.


Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk Using SPE Cleanup and LC/MS Analysis

Fast method for the cleanup, analysis, and quantification of aflatoxin M1 in milk samples to EU regulatory standards (0.025 μg/kg).

LC/MS Analysis of Aflatoxin M1 in Milk on Ascentis® Express HILIC after SPE using Supel™ Tox AflaZea, Quantifying Picogram Concentrations

Protocol for LC/MS Analysis of Aflatoxin M1 in Milk on Ascentis® Express HILIC after SPE using Supel™ Tox AflaZea, Quantifying Picogram Concentrations

LC/MS/MS Analysis of Aflatoxins in Hops on Ascentis® Express 2.7 μm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE

LC/MS/MS Analysis of Aflatoxins in Hops on Ascentis® Express 2.7 μm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE

See All

Related Content

Supel™ Tox SPE Products for Mycotoxin Analysis

Supel™ Tox video demonstrates how this SPE product accomplishes mycotoxin sample prep in fewer steps, with better reproducibility and higher sample throughput than IAC.

Mycotoxin Analysis in Food and Feed: Detecting the Fungus Among Us

Mycotoxin chemistry is diverse with some toxins being relatively harmless and some very dangerous like aflatoxins, citrinin, ergot alkaloids fumonisins. For all Food & Beverage manufacturers and testers, find a detailed list of products for each step of your mycotoxin analysis workflow.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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