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58298

SUPELCOSIL LC-18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 (octadecyl) phase
Pore size:
120 Å
Matrix:
silica gel, spherical particle platform
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Product Name

SUPELCOSIL LC-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L1

Quality Segment

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

11.0% carbon loading

parameter

≤70 °C temp. limit, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (RP18), C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

General description

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Application

SUPELCOSIL LC-18 HPLC Column may be used for the separation of:
  • Anthocyanins, flavonols, and carbohydrates in Tropea red onions (Allium cepa L.) during their determination by high performance liquid chromatography (HPLC) as well as HPLC coupled to mass spectrometry (MS) equipped with electrospray ionization (ESI) source.
  • Sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice samples during their determination by semipreparative reversed phase HPLC.
  • Aflatoxin M1 in milk and cheese samples during its determination by HPLC with post-column derivatization and fluorescence detection.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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1 of 1

This Item
548495793457935
L × I.D.

25 cm × 4.6 mm

L × I.D.

25 cm × 21.2 mm

L × I.D.

15 cm × 2.1 mm

L × I.D.

25 cm × 2.1 mm

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1


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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Related Content

Separation of δ-Tocopherol, analytical standard; Retinyl acetate; (+)-γ-Tocopherol; α-Tocopherol, ≥95.5%; Retinol; Vitamin A (acetate), meets USP testing specifications.


Flavonoid and carbohydrate contents in Tropea red onions: effects of homelike peeling and storage.
Gennaro L, et al.
Journal of Agricultural and Food Chemistry, 50(7), 1904-1910 (2002)
Vagish Dwibedi et al.
Biotechnology letters, 43(3), 627-644 (2020-11-08)
The present investigation primarily focusses on enhancement of resveratrol production by endophytic production from the endophytic fungus, Arcopilus aureus via one variable at a time approach (OVAT) followed by statistical approach using response surface methodology (RSM). The paper also highlights
Characterization of sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice.
Wen L, et al.
Journal of Agricultural and Food Chemistry, 47(3), 850-853 (1999)



Global Trade Item Number

SKUGTIN
C6013-25G04061833076057
C6013-5G04061833076064
5829804061838549785

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is part No.: 58298 available as "4.6mm ID" or "4.0 mm ID" ? As per product description it is "4.6mm ID" while as per SDS description it shows "4.0 mm ID".

    1 answer
    1. The inner diameter of this column is 4.6 mm.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Is this column comparable to the Phenomenex Luna C18 4.6 X 250mm, 5 micron

    1 answer
    1. Yes. This column is comparable to the Phenomenex Luna C18 4.6 X 250mm, 5 micron, with respect to dimensions and phase type (USP L1).

      Helpful?

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