Millicell® cell culture inserts are designed to maximize biological relevance and user convenience in cell culture applications. In vivo, cells live in a three-dimensional environment and can access nutrients from all directions. In contrast, traditional plastic culture plates force cells to grow on a smooth, two-dimensional surface, leading to flattened nuclei and poor function. Millicell® permeable inserts and plates feature a design that allows access to both the basolateral and apical surfaces of cell layers. This promotes natural, multidimensional growth and provides more relevant in vitro models, leading to more biologically relevant results for applications including toxicity screening, primary and secondary screening, cell signaling, cell proliferation, transport assays, and ADME/Tox drug safety studies.
Cell culture with Millicell® hanging inserts
Millicell® hanging inserts make it possible to study both apical and basal surfaces of the cell monolayer, and are excellent tools for coculture, as there is no disturbance of the outer basolateral cell culture by the presence of insert feet.
Millicell® Standing Inserts are free-standing inserts that can rest at the bottom of cell culture plate wells and other vessels, elevated by insert feet. These standing inserts promote the natural growth of suspension and adherent cell lines and are available in three different optimized membranes tailored for specific needs.
Millicell® Organotypic Standing Inserts are designed for superior study of three-dimensional explant structures.
Millicell®-24 and Millicell®-96 Plate Assemblies enable cell growth, feeding, and analysis in one membrane-bottom plate optimized to grow and sustain high-integrity cell monolayers. These plates can be used manually or with automated cell seeding, feeding, and washing systems. Their thoughtful design improves function with seed-and-feed systems, automated liquid handling systems, and transepithelial electrical resistance (TEER) measurement instruments.
Millicell® ULA plates are reliable 3D cell culture tools that promote scaffold free self-assembly of spheroids. The 96-well U bottom plates are compatible with analysis and brightfield and fluorescence imaging as the wells have high optical clarity. The non-cytotoxic, stable, and non-adhesion surface of Millicell® ULA plates support high throughput spheroid assembly.
Millicell® Microwell plates are ready-to-use 3D culture tools for high throughput and reproducible organoid culture. Based on an array of ultra-dense U-bottom shaped microwells (μW), single organoids are generated in each microcavity and grown in a pre-cast hydrogel without a solid extracellular matrix. With multiple configurations and microwell diameters available, Millicell® Microwell plates fit your organoid application needs.
Recommended membrane material and pore sizes for Millicell® cell culture inserts and plates are based on cell type and application.
Millicell® EZ Slides can be used to culture, fix, stain and view your cells all in one device. There’s no need to tediously move cover slips from your culture dish to a slide, or to remove the media chamber from the slide prior to fixing or staining. Easily remove the chamber structure without worrying about breaking slides or ruining cell layers. Acquire data simply and quickly with Millicell® EZ Slides.
Millicell® HY Flasks are innovative multilayer cell culture flasks that support uniform, consistent cell growth for high cell recovery. Manufactured with multiple identical layers, these flasks maximize cell culture surface area for the highest yields, while saving incubator space.
Article: Alternative High-Yield Cell Culture Systems
Brochure: Multiply Your Success MultiScreen® and Millicell® Filter Plates for Assay Development
Data Sheet: Millicell® Inserts and Plates
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