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Antonella Amore et al.
Microbial cell factories, 11, 164-164 (2012-12-27)
The use of lignocellulosic materials for second generation ethanol production would give several advantages such as minimizing the conflict between land use for food and fuel production, providing less expensive raw materials than conventional agricultural feedstock, allowing lower greenhouse gas
Laura Sutarlie et al.
Journal of colloid and interface science, 411, 76-81 (2013-10-12)
Cellulase is an important enzyme for hydrolyzing cellulose to form glucose. To recycle cellulase after the reaction, cellulase is often immobilized on solid supports but its activity is also compromised. In this study, we show a new hybrid cellulase aggregate
Takao Arimori et al.
Journal of synchrotron radiation, 20(Pt 6), 884-889 (2013-10-15)
The saccharification process is essential for bioethanol production from woody biomass including celluloses. Cold-adapted cellulase, which has sufficient activity at low temperature (<293 K), is capable of reducing heating costs during the saccharification process and is suitable for simultaneous saccharification
Immacolata Venditto et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 69(Pt 2), 191-194 (2013-02-07)
The rumen anaerobic cellulolytic bacterium Eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. One of these enzymes, endoglucanase Cel5A, comprises a tandemly repeated carbohydrate-binding module (CBM65) fused to
Vittoria Locato et al.
Journal of experimental botany, 66(10), 2991-3000 (2015-04-22)
Ophiobolin A, a tetracyclic sesterpenoid produced by phytopathogenic fungi, is responsible for catastrophic losses in crop yield but its mechanism of action is not understood. The effects of ophiobolin A were therefore investigated on the growth and redox metabolism of
Mitchell Lever et al.
Environmental technology, 34(1-4), 471-475 (2013-03-28)
The production of crude cellulase in solid-state fermentation was simplified by using environmental selection pressures as an alternative to specialized strains and by re-using cellulose-to-ethanol fermentation substrate for cellulase production. The performance of wild strains of Trichoderma viride isolated from
Mark A Currie et al.
The Journal of biological chemistry, 288(11), 7978-7985 (2013-01-24)
Clostridium thermocellum produces the prototypical cellulosome, a large multienzyme complex that efficiently hydrolyzes plant cell wall polysaccharides into fermentable sugars. This ability has garnered great interest in its potential application in biofuel production. The core non-catalytic scaffoldin subunit, CipA, bears
Margaret Buchanan et al.
BMC plant biology, 12, 235-235 (2012-12-13)
Endo-(1,4)-β-glucanase (cellulase) glycosyl hydrolase GH9 enzymes have been implicated in several aspects of cell wall metabolism in higher plants, including cellulose biosynthesis and degradation, modification of other wall polysaccharides that contain contiguous (1,4)-β-glucosyl residues, and wall loosening during cell elongation.
Wei Weiqi et al.
Bioresource technology, 128, 725-730 (2012-12-25)
Combination of liquid hot water pretreatment (LHWP) and wet disk milling (WDM) was investigated in this study to enhance the sugar recovery yield both in prehydrolyzate and enzymatic hydrolyzate. The results show that WDM with LHWP at 180 °C for
Jin Zhou et al.
Bioresource technology, 100(2), 819-825 (2008-09-06)
To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven
David N Bryant et al.
Bioresource technology, 128, 765-768 (2012-12-12)
Dielectric spectroscopy (DS) has been used to monitor the simultaneous saccharification and fermentation of lignocellulosic biomass by measuring its dielectric state. However, it is unknown whether following steam explosion (SE) pre-treatment, lignocellulose would still maintain a dielectric state, and, if
Orsolya Erzsébet Szabó et al.
Carbohydrate polymers, 98(2), 1483-1489 (2013-09-24)
A commercial acidic cellulase enzyme complex was chosen in order to gain detailed information about the effect of low-frequency ultrasound (horn at 40 kHz) on the enzyme activity. The performance of the enzyme under sonication was also evaluated in a
Akihiko Nakamura et al.
Journal of synchrotron radiation, 20(Pt 6), 859-863 (2013-10-15)
Neutron protein crystallography (NPC) is a powerful tool for determining the hydrogen position and water orientation in proteins, but a much larger protein crystal is needed for NPC than for X-ray crystallography, and thus crystal preparation is a bottleneck. To
Clara Bourbousse et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(21), E2836-E2844 (2015-05-13)
The spatial organization of chromatin can be subject to extensive remodeling in plant somatic cells in response to developmental and environmental signals. However, the mechanisms controlling these dynamic changes and their functional impact on nuclear activity are poorly understood. Here
Elizabeth A Znameroski et al.
The Journal of biological chemistry, 289(5), 2610-2619 (2013-12-18)
Neurospora crassa colonizes burnt grasslands and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source to cellulose, N. crassa dramatically up-regulates expression and secretion of genes encoding lignocellulolytic enzymes. However, the means by
Cellulases: Cooperative biomass breakdown.
Kiyohiko Igarashi
Nature chemical biology, 9(6), 350-351 (2013-04-09)
Aditya Bhalla et al.
Bioresource technology, 128, 751-759 (2012-12-19)
Second-generation feedstock, especially nonfood lignocellulosic biomass is a potential source for biofuel production. Cost-intensive physical, chemical, biological pretreatment operations and slow enzymatic hydrolysis make the overall process of lignocellulosic conversion into biofuels less economical than available fossil fuels. Lignocellulose conversions
Flávio R Rosseto et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 69(Pt 2), 137-140 (2013-02-07)
Cellulases, such as endoglucanases, exoglucanases and β-glucosidases, are important enzymes used in the process of enzymatic hydrolysis of plant biomass. The bacteria Xanthomonas campestris pv. campestris expresses a large number of hydrolases and the major endoglucanase (XccEG), a member of
Alexander V Gusakov
Science (New York, N.Y.), 344(6184), 578-578 (2014-05-09)
Brunecky et al. (Reports, 20 December 2013, p. 1513) compared the cellulolytic activity of bacterial multimodular cellulase CelA with fungal Cel7A (cellobiohydrolase I from Trichoderma reesei). If more active Cel7A from another fungus were used as a reference enzyme under
Arpan Das et al.
Polish journal of microbiology, 62(1), 31-43 (2013-07-09)
This study was aimed to evaluate the pattern of cellulase biosynthesis from Aspergillusfumigatus ABK9 under submerged fermentation. Production was increased concomitantly with fungal growth up to 72 h and reached maximum (Xmax -6.72 g/l) with specific growth rate (mu max)
Guosheng Xie et al.
PloS one, 8(1), e50171-e50171 (2013-01-12)
Plant glycoside hydrolase family 9 (GH9) comprises typical endo-β-1,4-glucanase (EGases, EC3.2.1.4). Although GH9A (KORRIGAN) family genes have been reported to be involved in cellulose biosynthesis in plants, much remains unknown about other GH9 subclasses. In this study, we observed a
Aboozar Kazemi et al.
Preparative biochemistry & biotechnology, 44(2), 107-118 (2013-10-25)
Cellulases are important glycosyl hydrolase enzymes, which break down cellulose to β-glucose. They have been used widely in biotechnological processing such as bioethanol production. In this work we studied maximizing cellulase production by Bacillus sp. BCCS A3 using response surface
Cheng Fan et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(9), 1113-1121 (2012-12-15)
To isolate and identify the cellulose-producing bacterium from fresh feces of healthy giant pandas, and characterize its cellulase production. A strain with high activity of cellulase was isolated and purified by carboxymethyl cellulose-Na (CMC-Na) medium. The morphological, physiological and biochemical
Xing-hua Li et al.
Molecular biology reports, 37(8), 3721-3728 (2010-03-03)
Cellulases catalyze the hydrolysis of cellulose which are mainly three types: endoglucanases, cellobiohydrolases and β-glucosidases. It can be used in converting cellulosic biomass to glucose that can be used in different applications such as production of fuel ethanol, animal feed
Diogo Robl et al.
BMC biotechnology, 13, 94-94 (2013-11-02)
There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry
Improved procedures for preparation and characterization of myrothecium cellulase. 2. Purification procedures.
Canadian journal of biochemistry and physiology, 41, 671-696 (1963-03-01)
F Xu et al.
Prikladnaia biokhimiia i mikrobiologiia, 47(1), 61-65 (2011-03-29)
The filamentous fungi Trichoderma species produce extracellular cellulase. The current study was carried out to obtain an industrial strain with hyperproduction of cellulase. The wild-type strain, Trichoderma viride TL-124, was subjected to successive mutagenic treatments with UV irradiation, low-energy ion
Mareike Monschein et al.
Bioresource technology, 128, 679-687 (2012-12-12)
Marked slow-down of soluble sugar production at low degree of substrate conversion limits the space-time yield of enzymatic hydrolysis of ligno-cellulosic materials. A simple set of kinetic descriptors was developed to compare reducing sugar release from pure crystalline cellulose (Avicel)
Paula Berenstecher et al.
Current biology : CB, 30(16), 3243-3251 (2020-07-04)
Photodegradation of aboveground senescent plant material (plant litter) due to exposure to solar radiation has been identified as a dominant control on carbon (C) loss in semi-arid ecosystems [1], upturning traditional models of C cycling based only on available moisture
Feng-Juan Xu et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 39(2), 235-239 (2014-04-26)
To prepare sagittatoside B with epimedin B Hydrolyzed from cellulase. With the conversion ratio as the index, the effects of pH value, temperature, reaction time, dosage of enzyme and concentration of substrates on the conversion ratio were detected. L9 (3(4))
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