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Applications:Advanced gene editing
Content Type:Technical Article
Showing 1-30 of 38 results
What Is ZFN Technology?
Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Tips for Cell Engineering Using Cas9-GFP CRISPR Plasmids
CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.
CRISPR/Cas-GFP Vectors for Rapid Expression Verification and Enrichment of Genome Edited Cells
View experimental data showing crispr/cas expression and enrichment using FACS
Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
TRC Design and pLKO.1 Vector FAQs
The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.
Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process
The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).
CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic the human brain transcriptome - not to mention the blood-brain barrier - make genetic perturbation analysis
Advanced RNAi Applications — in vivo FAQs
We answer your frequently asked questions about in vivo RNAi applications.
Targeted Genome Editing Using Engineered Zinc Finger Nucleases
Targeted genome editing with Zinc finger nucleases (ZFNs) has a wide variety of applications, and three key modes of action have been a focus thus far: targeted gene knockouts, targeted gene integration, and targeted gene correction.
Precision Genetic Modifications
Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address
Viral Invaders
Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing.
Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Small Molecule CRISPR Enhancers
The CRISPR-Cas9 system is an RNA-guided genome-editing tool that provides researchers a simple, easy, and quick way to modify the genomes of various organisms.
How Do ZFNs Work?
CompoZr Zinc Finger Nucleases (ZFNs) are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections.
Long Oligos
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
Methods for Enhancing Lentiviral Transduction Efficiency
An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.
Zinc Finger Nuclease Frequently Asked Questions
Zinc Finger Nuclease - frequently asked questions
shRNA Gene Families Sets
The MISSION® shRNA Library gene family sets are collections of genes related to specific cellular/molecular pathways documented in NCBI, Gene Ontology (GO), Protein Lounge, and Ingenuity Systems. Our Bioinformatics team has methodically mapped all TRC shRNA clones to each set.
Genome Engineering with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Genome Editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Watch our video.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
CRISPR/CAS9 Gene Editing Protocol for Human Induced Pluripotent Stem Cells (iPSCs)
This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs).
NitroGENIUS: Engineering E. coli to Fix Nitrogen and Regulating Transcription with Light
Endosymbiotic theory states that chloroplasts in plants originally came from a symbiotic relationship between prokaryotes.
CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein
Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.
Prime Editing – An Exciting Combination of Natural and Engineered RNA-guided Genome Editing
Prime Editing is a novel variation on CRISPR systems which expands the guide RNA’s responsibility to serve two purposes: (1) to guide Cas9 to a targeted genomic location, and (2) to serve as an RNA template to copy new sequences
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
MISSION shRNA Lentiviral Transduction
MISSION® shRNA Lentiviral Transduction application data
Bacterial Transformation
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
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