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Competent Cell Protocols
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
Escort™ III Transfection Reagent Protocol
The product bulletin providin detailed use protocol for easy DNA transfection.
Co-transfection of Plasmid DNA
Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Lipid Transfection Optimization Protocol
Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.
BL21(DE3) Electrocompetent Cells
BL21(DE3) Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note that alternate settings result in transformation
Restriction Enzyme Digest Protocol
Restriction enzyme collection has been optimized for digestion using five unique buffers.
pGEX Vectors
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
T4 DNA Ligase (Rapid) Protocol
T4 DNA Ligase for joining blunt ends of DNA and RNA.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.
OverExpress™ Electrocompetent Cells
OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.
x-tracta Gel Extraction Tool
A quick, clean and easy to use alternative to using a scalpel or razor blade for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis.
OverExpress™ Chemically Competent Cells
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Dephosphorylation Procedures for DNA and Proteins
Dephosphorylation of DNA using Calf Intestinal Alkaline Phosphatase, Shrimp Alkaline Phospha, Bovine Intestinal Alkaline Phosphatase
Ligation of Insert to pGEX DNA
This page describes ligation of insert to pGEX DNA using Cytiva products.
EnPresso® B Protocol with Video
EnPresso B is a pre-sterilized growth system designed to increase the yield of functional protein from E. coli-based expression systems. EnPresso growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by
Ligation of Insert to pGEX DNA
This page describes ligation of insert to pGEX DNA using Cytiva products.
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
X-tremeGENE HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Universal Transfection Reagent Protocol
Our Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. This is a fast and easy protocol is compatible
Blue-White Select™ Screening Reagent
Blue/white color selection is a routine technique employed by molecular biologists. This technique simplifies the differentiation between colonies/plaques that contain a cloning vector without an insert and those that contain a vector harboring an insert of interest.
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Preparing Cells for Electroporation
Preparing Cells for Electroporation
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
SIG10 Chemically Competent Cells
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
Klenow Enzyme Protocol
Protocols of standard assays for partial and complete filling in can be found in LabFaqs.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
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