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How to make and use gradients of Percoll
In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
ECM Coating Protocols for Millicell®
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Coating Tissue Culture Plates with Vitronectin
Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.
Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
Rat Dermal Fibroblasts (RDF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Laminin Coating Protocol for Cell Culture
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Cryopreservation of Cell Lines
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Trypsin Inhibitors
Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.
Testing Cells for Bacterial and Fungal Contamination
Cell culture protocol for testing cell lines for bacterial and fungal contamination.
StableCell™ Trypsin Solutions
StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.
Cell Culture Protocol 1: Proper Aseptic Technique and Sterile Handling of Cells
Cell culture protocol for proper aseptic technique to maintain a sterile environment and to prevent cell culture contamination. Free ECACC handbook download.
Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts
This technical article covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Powdered Media Preparation Instructions
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Gelatin Coating Protocol for Cell Culture
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
Poly-L-Lysine Cell Attachment Protocol
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Trypsin Cell Dissociation Protocol
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Subculture of Adherent Cell Lines
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
HL-1 Cells
HL-1 cell research support includes culture plates, media, and Cryovials, providing a complete offering for research needs.
Dextran
Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.
Fibronectin Coating Protocol
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
Installation & Use of Milli-Q® SQ 2Series Water Purification Systems
View instructional videos and download User Manuals for Milli-Q® SQ 2Series lab water systems. You’ll be up and running in no time. Buy online systems, accessories, cartridges and services.