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Protein labeling and modification

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Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Introduction to Target Identification with Hyas and Dione Kits
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
Phosphorylation Analysis Tools
Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Unnatural Amino Acids for Peptide Synthesis
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Protein Deglycosylation
Protein Deglycosylation: PNGase F Proteomics Grade, Native Protein Deglycosylation Kit, Enzymatic Protein Deglycosylation Kit, and ProteoProfile Trypsin In-Gel Digest Kit
Gold Nanoparticles: Properties and Applications
Gold (Au) nanoparticles have tunable optical and electronic properties and are used in a number of applications including photovoltaics, sensors, drug delivery & catalysis.
Residual Peroxide Determination in Food Packaging
ECM microenvironment shapes cell processes; engineering advances needed for complex control in cell and tissue studies.
Designing Peptides
Consider sequence length, solubility, and stability when designing peptides for custom synthesis.
Post Translational Modification
Protein modifications are crucial for disease study. Analysis methods are key.
Glycoprofile™ Labeling Kits
Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.
Solid Phase Synthesis
Peptides synthesized using FMOC or BOC chemistry on PEG-Polystyrene support resin, then cleaved, precipitated, and lyophilized.
Peptidoglycan Structure and Biosynthesis
Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.
O Linked Glycans
O-Linked glycans attachment to peptides involves various residues, particularly GalNAc, known as mucin-type glycans.
Phosphorylation Basics
Explore phosphorylation types converting ADP to ATP, comparing oxidative and substrate-level phosphorylation with explanatory diagrams.
Glycosyltransferases: Tools for Synthesis and Modification of Glycans
Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.
Understand the role of glycotransferases, see strategies to prevent glycotransferase enzyme inhibition, and find glycosyltransferase kits and products.
Self-Assembled Monolayers: Advantages of Pure Alkanethiols
We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Applications of Y-Shape Peg Derivatives for Drug Delivery
The immune system protects the body from disease by resisting the invasion of foreign molecules into its cells, for example peptides and proteins are hydrolyzed by proteolytic enzymes; nucleic acids are hydrolyzed by nucleases; and most small molecules are eliminated
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling
We offer protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes. Both series of kits provide an easy and reliable way to fluorescently label purified proteins, enzymes, and antibodies.
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