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Showing 1-30 of 44 results
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up
This page covers how and when to use Sepharose XL media for purification of proteins.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
Performing a Separation of Fibronectin with Gelatin Sepharose® 4B
This page shows how to separate fibronectin by affinity chromatography using Gelatin SepharosePerforming a Separation of Fibronectin with Gelatin Sepharose® 4B from Cytiva.
Capto Adhere ImpRes
Capto adhere ImpRes is a cost-effective and flexible chromatography medium from Cytiva, designed for high-resolution polishing of MAbs.
Purification Using MBPTrap™ HP Columns
This page shows how to purify MBP-tagged proteins using MBPTrap HP columns from Cytiva.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.
Affinity Chromatography in a Purification Strategy (CIPP)
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
Purification or Removal of Phosphorylated Biomolecules
This page shows how to Purify or remove phosphorylated biomolecules with a TiO2 Mag Sepharose from Cytiva.
Performing a Purification with Calmodulin Sepharose 4B
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.
Coupling Small Ligands Through Carboxyl Groups via a Spacer Arm
Ligands are coupled in a simple one-step procedure in the presence of a coupling reagent, carbodiimide. The carbodiimides may be regarded as anhydrides of urea.
Performing a Separation with Sephadex®
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Capto Core 700
Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Reverse Phased Chromatography (RPC) in Practice
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
Removal of Detergents from Biological Product Matrices
The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.
Packing a Column and Performing a Separation with Sephadex® LH-20
This page shows how to perform a separation with a Sephadex LH-20 column from Cytiva which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.
Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum...
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Characteristics of Glutathione Sepharose® Products
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Performing a Separation with Sephacryl®
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
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