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Bacteroides-Derived Sphingolipids Are Critical for Maintaining Intestinal Homeostasis and Symbiosis.

Cell host & microbe (2019-05-10)
Eric M Brown, Xiaobo Ke, Daniel Hitchcock, Sarah Jeanfavre, Julian Avila-Pacheco, Toru Nakata, Timothy D Arthur, Nadine Fornelos, Cortney Heim, Eric A Franzosa, Nicki Watson, Curtis Huttenhower, Henry J Haiser, Glen Dillow, Daniel B Graham, B Brett Finlay, Aleksandar D Kostic, Jeffrey A Porter, Hera Vlamakis, Clary B Clish, Ramnik J Xavier

Sphingolipids are structural membrane components and important eukaryotic signaling molecules. Sphingolipids regulate inflammation and immunity and were recently identified as the most differentially abundant metabolite in stool from inflammatory bowel disease (IBD) patients. Commensal bacteria from the Bacteroidetes phylum also produce sphingolipids, but the impact of these metabolites on host pathways is largely uncharacterized. To determine whether bacterial sphingolipids modulate intestinal health, we colonized germ-free mice with a sphingolipid-deficient Bacteroides thetaiotaomicron strain. A lack of Bacteroides-derived sphingolipids resulted in intestinal inflammation and altered host ceramide pools in mice. Using lipidomic analysis, we described a sphingolipid biosynthesis pathway and revealed a variety of Bacteroides-derived sphingolipids including ceramide phosphoinositol and deoxy-sphingolipids. Annotating Bacteroides sphingolipids in an IBD metabolomic dataset revealed lower abundances in IBD and negative correlations with inflammation and host sphingolipid production. These data highlight the role of bacterial sphingolipids in maintaining homeostasis and symbiosis in the gut.

Product Number
Product Description

L-Alanine-2,3,3,3-d4, 98 atom % D
Ampicillin sodium salt, powder or crystals, BioReagent, suitable for cell culture
Ninhydrin, ACS reagent
Gentamicin solution, 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture
D-(−)-Fructose, ≥99%
D-erythro-Dihydrosphingosine, ≥98%
C12 Sphingosyl PE (d17:1/12:0), Avanti Polar Lipids 860529P, powder
Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer