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Acetylcholinesterase immobilized on magnetic beads for pesticides detection: application to olive oil analysis.

Sensors (Basel, Switzerland) (2012-09-13)
Najwa Ben Oujji, Idriss Bakas, Georges Istamboulié, Ihya Ait-Ichou, Elhabib Ait-Addi, Régis Rouillon, Thierry Noguer
ABSTRACT

This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.

MATERIALS
Product Number
Brand
Product Description

Supelco
Malathion solution, 100 μg/mL in cyclohexane, PESTANAL®, analytical standard
Supelco
Malathion, PESTANAL®, analytical standard
Supelco
Malaoxon, PESTANAL®, analytical standard
Supelco
Methidathion, PESTANAL®, analytical standard
Supelco
Malathion, certified reference material, TraceCERT®
Supelco
Omethoate, PESTANAL®, analytical standard
Malathion impurity B, European Pharmacopoeia (EP) Reference Standard