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Effects of sterilization and storage on the properties of ALP-grafted biomaterials for prosthetic and bone tissue engineering applications.

Biomedical materials (Bristol, England) (2012-09-14)
S Ferraris, G Pan, C Cassinelli, L Mazzucco, E Vernè, S Spriano
ABSTRACT

Grafting of the biomaterial surfaces with biomolecules is nowadays a challenging research field for prosthetic and bone tissue engineering applications. On the other hand, very few research works investigate the effect of the sterilization processes on the properties of functionalized biomaterials. In this study, the effects of different sterilization techniques (e.g. gamma and electron beam irradiation, ethylene oxide) on the enzymatic activity of bioactive glasses and Ti6Al4V grafted with alkaline phosphatase (ALP) have been analyzed. Sterility maintenance and in vitro bioactivity of the sterilized surfaces have also been investigated. Finally the effect of packaging and storage conditions has been considered.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ethylene oxide solution, certified reference material, 50 mg/mL in methanol
Sigma-Aldrich
Ethylene oxide solution, 2.5-3.3 M in THF