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Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides.

Journal of lipid research (1969-05-01)
P Belfrage, M Vaughan
ABSTRACT

Oleic acid has a partition coefficient, upper phase/lower phase, of 1.9 (22 degrees C) in the liquid-liquid partition system described herein. Tri-, di-, and monoolein are found almost exclusively in the lower (organic) phase. Oleic acid can be quantitatively removed from mixtures of triglyceride and partial glycerides by means of this partition system under conditions resembling those in a lipase assay.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)