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Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.

Carbohydrate polymers (2014-05-13)
Zhen-Bin Wang, Juan-Juan Pei, Hai-Le Ma, Pan-Fu Cai, Jing-Kun Yan
ABSTRACT

Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively. PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents. PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios. All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner. The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities. The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities. PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.

MATERIALS
Product Number
Brand
Product Description

Xylose, European Pharmacopoeia (EP) Reference Standard
Galactose, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D-(−)-Arabinose, ≥98%
Sigma-Aldrich
D-(+)-Mannose, powder, BioReagent, suitable for cell culture
Supelco
D-(+)-Glucose, analytical standard
Sigma-Aldrich
D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
Sigma-Aldrich
D-(+)-Glucose, tested according to Ph. Eur.
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
Sigma-Aldrich
D-(+)-Galactose, meets analytical specification of Ph. Eur., BP
Millipore
D-(−)-Arabinose, suitable for microbiology, ≥99.0%
Sigma-Aldrich
D-(+)-Glucose, meets analytical specification of Ph. Eur., BP, anhydrous
Sigma-Aldrich
D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
D-(+)-Mannose, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
Millipore
D-(+)-Mannose, suitable for microbiology, ≥99%
Millipore
D-(+)-Galactose, suitable for microbiology, ≥99.0%
Millipore
D-(+)-Mannose, ≥99.0% (sum of enantiomers, HPLC), suitable for microbiology
Sigma-Aldrich
D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Glucose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Galactose, ≥98%
Sigma-Aldrich
D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
D-(+)-Galactose, ≥99%
Sigma-Aldrich
Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
Sigma-Aldrich
Hydrogen peroxide solution, purum p.a., ≥35% (RT)
Supelco
Galactose, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D-(+)-Mannose, wood, ≥99%
Sigma-Aldrich
D-(+)-Mannose, synthetic, ≥99%
SAFC
Galactose, plant-derived
Sigma-Aldrich
D-(+)-Glucose, ACS reagent
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC)