Merck
  • Home
  • Search Results
  • Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Nanoscale research letters (2014-11-12)
Pierre-Yves Collart-Dutilleul, Ivan Panayotov, Emilie Secret, Frédérique Cunin, Csilla Gergely, Frédéric Cuisinier, Marta Martin
ABSTRACT

The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments (filopodia) formation. In this work, we report on the use of porous silicon (pSi) scaffolds to mimic the ECM of mesenchymal stem cells from the dental pulp (DPSC) and breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, and scanning electron microscopy (SEM) results show that pSi promoted the appearance of lateral filopodia protruding from the DPSC cell body and not only in the lamellipodia area. The formation of elongated lateral actin filaments suggests that pores provided the necessary anchorage points for protrusion growth. Although MCF-7 cells displayed a lower presence of organized actin network on both pSi and nonporous silicon, pSi stimulated the formation of extended cell protrusions.

MATERIALS
Product Number
Brand
Product Description

Carbon - Vitreous, foil, 100x100mm, thickness 6.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 5.0mm, glassy carbon
Carbon - Vitreous, foil, 8mm disks, thickness 1.0mm, glassy carbon
Carbon - Vitreous, foam, 275x330mm, thickness 3.2mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, rod, 100mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, foam, 275x330mm, 0.05g.cmué, porosity 96.5%, 24 pores/cm
Carbon - Vitreous, foam, 150x150mm, thickness 2.5mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, foil, 25x25mm, thickness 0.5mm, glassy carbon
Carbon - Vitreous, foam, 300x300mm, thickness 20mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, foil, 10x10mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 3.0mm, glassy carbon
Carbon - Vitreous, foil, 25x25mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, foam, 300x300mm, thickness 30mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, rod, 200mm, diameter 10mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 5.0mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 2.0mm, glassy carbon
Carbon - Vitreous, foil, 10x10mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, tube, 100mm, outside diameter 10mm, inside diameter 3mm, wall thickness 3.5mm, glassy carbon
Carbon - Vitreous, foil, 50x50mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, tube, 50mm, outside diameter 10mm, inside diameter 3mm, wall thickness 3.5mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 7.0mm, glassy carbon
Carbon - Vitreous, foil, 50x50mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, rod, 50mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 3.0mm, glassy carbon
Carbon - Vitreous, foil, 8x8mm, thickness 0.5mm, glassy carbon
Carbon - Vitreous, rod, 5mm, diameter 3.0mm, glassy carbon
Sigma-Aldrich
Ethanol, purum, fine spirit, denaturated with 2% 2-butanone, F25 MEK1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1