Analyze Iron (Total) in Plant Material

The Spectroquant^® Iron test allows the accurate quantification of iron ions in various waters. All iron ions are reduced to iron(II) ions by ascorbic acid. In a thioglycolate-buffered medium, these react with a triazine derivative to form a red-violet complex that is measured photometrically.

Materials

• Spectroquant^® VIS Spectrophotometer Prove 100 (Product No. 173016), or Spectroquant^® UV/VIS Spectrophotometer Prove 300 (Product No. 173017), or Spectroquant^® UV/VIS Spectrophotometer Prove 600 (Product No. 173018), or Spectroquant^® Photometer NOVA 30 (Product No. 109748), or Spectroquant^® Photometer NOVA 60 (Product No. 109751), or Spectroquant^® Colorimeter Move 100 (Product No. 173632)
• Rectangular cells 10 mm (Product No. 114946), and/or Rectangular cells 20 mm (Product No. 114947), and/or Rectangular cells 50 mm (Product No. 114944 )
  
• Spectroquant^® Iron Cell Test (Product No. 114549)
• Spectroquant^® Iron Cell Test* (Product No. 114896)
• Spectroquant^® Iron Test (Product No. 114761)
• Spectroquant^® Iron Test (Product No. 100796)
• Nitric acid 65 % for analysis (Product No. 100456)
• Sulfuric acid 95 - 97% for analysis (Product No. 100731)
• Perchloric acid 70% for analysis (Product No. 100519)
• Water for analysis (Product No. 116754)

*not compatible with Move 100

Plant Material Sample Preparation

Ground the plant material finely and pass through a sieve (mesh 0.4 - 0.7 mm) and dry the material at 105 °C for 3 hours.

Add 1.0 g of the dried material to a mixture of 1.5 mL nitric acid 65 %, 0.5 mL perchloric acid 70 %, 1 mL sulfuric acid 95 - 97 % and heat until white fumes are envolved (fume cupboard!). Dissolve the residue, after cooling, in water for analysis and fill up to the mark in a 100-mL standard volumetric flask and mix well.

Analysis

Determine iron content with the above mentioned test kits.

Calculation