The Spectroquant® Iron test allows the accurate quantification of iron ions in various waters. All iron ions are reduced to iron(II) ions by ascorbic acid. In a thioglycolate-buffered medium, these react with a triazine derivative to form a red-violet complex that is measured photometrically.
*not compatible with Move 100
Ground the plant material finely and pass through a sieve (mesh 0.4 - 0.7 mm) and dry the material at 105 °C for 3 hours.
Add 1.0 g of the dried material to a mixture of 1.5 mL nitric acid 65 %, 0.5 mL perchloric acid 70 %, 1 mL sulfuric acid 95 - 97 % and heat until white fumes are envolved (fume cupboard!). Dissolve the residue, after cooling, in water for analysis and fill up to the mark in a 100-mL standard volumetric flask and mix well.
Determine iron content with the above mentioned test kits.
|Iron (total) content in mg/kg Fe =||Analysis value in mg/L Fe x 100|
|Weight of sample in g|
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