OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note, alternate setting result in 20-50% lower efficiencies. Typical time constants are 3.5 to 4.5 msec.
To ensure successful transformation results, the following precautions must be taken: