These mixed cellulose ester membrane discs are a convenient, high recovery way to dialyze micro-volumes of protein or nucleic acid solutions (<100 µL). With the “drop dialysis” technique, a floating filter disc replaces the dialysis sac.
Recoveries of DNA and protein samples have been reported in excess of 98%.1
Drop Dialysis Technique
- Fill the bottom of a Petri dish with buffer.
- Float the membrane filter disc on the surface of the buffered solution.
- Deposit 5–100 µL of sample on the center of the membrane. Place a tight-fitting lid on the Petri dish to prevent evaporation.
Marker dyes may help in determining the dialysis time required. Most samples are dialyzed in less than 30 minutes.
- Recover the desalted sample.
“V” Series Membranes