These products are available in the US, Canada, and select European countries.
Mesenchymal Stem Cells (MSC), also called Mesenchymal Stromal Cells, are self-renewing multipotent cells that can differentiate into diverse cell types. MSC have been shown to differentiate in vitro into adipocytes, chondrocytes, osteoblasts, myocytes, and ß-pancreatic islet cells. MSC can also transdifferentiate into neuronal cells and hepatocytes.
PromoCell® Mesenchymal Stem Cells are produced from normal human tissues of varied origin including bone marrow, umbilical cord matrix, and adipose tissue. Differentiation of MSC into adipocytes, osteoblasts, chondrocytes, and neuronal lineages can be performed using the Mesenchymal Stem Cell Differentiation Media. Shortly after isolation, all Mesenchymal Stem Cells are cryopreserved using proprietary, serum-free freezing medium, Cryo-SFM. Thawing and seeding the cells constitutes passage 2 (P2). Each cryovial contains at least 500,000 viable cells when thawed. Human Mesenchymal Stem Cells are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days.
Mesenchymal Stem Cell Media was developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) isolated from bone marrow, the umbilical cord matrix (Wharton´s Jelly), or adipose tissue. Mesenchymal Stem Cell Media is available as Medium (ready-to-use) and consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium constitutes the complete Medium.
Mesenchymal Stem Cell Media contains all of the growth factors and supplements needed for the optimal expansion and directed differentiation of human mesenchymal stem cells. The Mesenchymal Stem Cell Growth Medium DXF, the MSC Chondrogenic Differentiation Medium, the MSC Chondrogenic Differentiation Medium w/o Inducers, and the MSC Neurogenic Differentiation Medium are all defined and serum-free. Mesenchymal Stem Cell Media do not contain antibiotics or antimycotics and are formulated for use in an incubator with an atmosphere of 5% CO2.
Note: The MSC Chondrogenic Differentiation Medium w/o Inducers must be supplemented by the user with adequate chondrogenic inducers.
The MSC Growth Medium DXF does not contain attachment and spreading factors. Therefore, culture vessels used with MSC Growth Medium DXF must be precoated with 10 μg/mL human fibronectin according to the manufacturers protocol. Alternatively, bovine fibronectin may be used.
Rigid quality control tests are performed for each lot of Human Mesenchymal Stem Cells.
All lots of PromoCell® Mesenchymal Stem Cell Media are subjected to comprehensive quality control tests using primary human mesenchymal stem cells. Each lot of MSC Growth Medium is validated for maintenance of multipotency, growth promoting activity, adherence rate, and typical morphology of the tested mesenchymal stem cells. Each lot of MSC Differentiation Media is tested for the capacity to induce directed differentiation of MSC to specific cell lineages. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Mesenchymal Stem Cells and Media System
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.