These products are available in the US, Canada, and select European countries.
The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. The diverse functions of epithelial cells include absorption, secretion, protection, transcellular transport, and sensation.
PromoCell® airway epithelial cells are isolated from normal human adult tissues of the large airways including nasal mucosa, trachea, and bronchi. Shortly after isolation, all human airway epithelial cells are cryopreserved at passage 2 (P2) using a proprietary serum-free freezing medium, Cryo-SFM. After thawing, each cryovial contains at least 500,000 viable cells. Human epithelial cells are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days.
The Airway Epithelial Cell Growth Medium is a serum-free growth medium optimized for the in vitro cultivation of epithelial cells from large air passages. The Airway Epithelial Cell Growth Medium is optimized for growing primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells. This medium is available in two options: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements), which gives the user full control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium results in the complete Airway Epithelial Cell Growth Medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack may also be purchased separately.
The Airway Epithelial Cell Growth Medium contains all of the growth factors and supplements needed for the optimal growth of human airway epithelial cells. These include epidermal growth factor, insulin, and transferrin (see the table below). Airway Epithelial Cell Growth Medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2
The following rigid quality control tests are performed for each lot of Airway Epithelial Cells:
All lots of PromoCell® Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth-promoting activity, adherence rate, and typical morphology of the tested cells. Approved in-house lots of Airway Epithelial Cell Growth Media are used as a reference. In addition, every lot of media is tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions, please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead, we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.