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HomeProtein PurificationAmicon® Pro Purification System – Protein Purification

Amicon® Pro Purification System – Protein Purification

The Amicon® Pro Purification System is an all-in-one centrifugal device for the protein purification workflow. Traditional protein purification is a long process with many steps and multiple tubes, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. Regardless of your workflow, this virtually touchless device delivers consistent, accurate sample preparation, resulting in reliable recovery, uncompromised purity and easy data generation.

The Amicon® Pro Purification System Workflow

Bind, wash, elute and/or concentrate your protein in this all-in-one centrifugal device.

The Amicon® Pro Purification System Workflow

Elute, Don't Dilute

Excellent Recovery, Regardless of Scale

elute dont elute

Figure 1.While three elution steps were required for the smaller spin columns (left), a single spin in the Amicon® Pro device was sufficient to recover > 90% of resin-bound protein in an average of 45 µL final volume*.

Excellent recovery

Figure 2.Protein recovered increases linearly with respect to packed resin volume, offering flexibility in experimental scale. Whether you are using a few microliters or a milliliter of resin, Amicon® Pro purification yields consistent recovery.

Compare the Amicon® Pro Workflow with Traditional Methods

a Process time excludes 60 minute incubation for both protocols.
b Gravity column process includes column setup, purification and assessment of eluted fractions.

Materials
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