Merck
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40718

Sigma-Aldrich

Diethyl pyrocarbonate

96% (NT)

Synonym(s):
Diethyl dicarbonate, DEPC, Diethyl oxydiformate, DEP, Ethoxyformic acid anhydride
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.22

Nível de qualidade

200

teor

96% (NT)

forma

liquid

cor

APHA: ≤20

índice de refração

n20/D 1.398 (lit.)
n20/D 1.398

pb

93-94 °C/18 mmHg (lit.)

solubilidade

95% ethanol: 4.5 g/10 mL, clear, colorless

densidade

1.121 g/mL at 20 °C
1.101 g/mL at 25 °C (lit.)

enviado em

wet ice

temperatura de armazenamento

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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Descrição geral

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Aplicação

Diethyl pyrocarbonate may be used in the following studies:
  • For the modification of histidyl residues in proteins.
  • As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
  • For the modification of linear and supercoiled plasmid DNAs.
  • As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
  • For cabethoxylation of histidine residues of actin.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Embalagem

25, 100 mL in poly bottle

Ações bioquímicas/fisiológicas

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Acute Tox. 4 Oral

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Ponto de fulgor (ºF)

156.2 °F - closed cup

Ponto de fulgor (ºC)

69 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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