608041

Sigma-Aldrich

L-Lysine-13C6,15N2 hydrochloride

99 atom % 15N, 99 atom % 13C, 95% (CP)

Sinônimo(s):
13C and 15N Labeled Lys, SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled lysine hydrochloride
Fórmula linear:
H215N(13CH2)413CH(15NH2)13CO2H · HCl
Número CAS:
Peso molecular:
190.59
Número MDL:
ID de substância PubChem:
NACRES:
NA.12

Nível de qualidade

200

pureza isotópica

99 atom % 13C
99 atom % 15N

teor

95% (CP)

atividade óptica

[α]25/D +20.7°, c = 2 in 5 M HCl

aplicação(ões)

bio NMR: suitable
protein expression: suitable

pf

263-264 °C (dec.) (lit.)

alteração de massa

M+8

SMILES string

Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O

InChI

1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;

InChI key

BVHLGVCQOALMSV-GSMNGUNQSA-N

Categorias relacionadas

Descrição geral

Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

Aplicação

Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.
  • L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
  • It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
  • It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).

Embalagem

1, 2 g in glass bottle
100, 250 mg in glass bottle
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

storage_class_code

11 - Combustible Solids

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture.
Tape CJ, et al.
Molecular and Cellular Proteomics, 13, 1866-1876 (2014)
Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells.
Yu C, et al.
Frontiers in Physiology, 7, 635-635 (2016)
Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach.
Ebner OA and Selbach M
Methods in Molecular Biology, 725, 315-331 (2011)
Stephanie D Kraft-Terry et al.
Journal of proteome research, 10(6), 2852-2862 (2011-04-20)
Dynamic interactions between human immunodeficiency virus-1 (HIV-1) and the macrophage govern the tempo of viral dissemination and replication in its human host. HIV-1 affects macrophage phenotype, and the macrophage, in turn, can modulate the viral life cycle. While these processes...
Harvey RA, et al.
Biochemistry (Lippincott's illustrated reviews) (2011)
Conteúdo relacionado
Protein structure provides valuable information that can be used to infer protein function. The study of protein structure and mapping of protein interactions, expression levels, and location enables the identification of disease biomarkers and potential drug targets for therapeutic treatment.
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