Merck
All Photos(2)

900892

Sigma-Aldrich

L-Azidohomoalanine hydrochloride

Synonym(s):
AHA
Empirical Formula (Hill Notation):
C4H8N4O2 · HCl
CAS Number:
Molecular Weight:
180.59

forma

powder or crystals

Nível de qualidade

100

reaction suitability

reaction type: click chemistry

pf

139 °C

temperatura de armazenamento

−20°C

Related Categories

Aplicação

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety. This compound can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. For example the azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI-MS analysis.

Pictogramas

Flame

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Self-react. C

Código de classe de armazenamento

5.2 - Organic peroxides and self-reacting hazardous materials

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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