Merck
Todas as fotos(4)

05-623

Sigma-Aldrich

Anti-RNA polymerase II Antibody, clone CTD4H8

clone CTD4H8, Upstate®, from mouse

Sinônimo(s):
Anti-POLR2, Anti-RPB1, Anti-hsRPB1, Anti-NEDHIB, Anti-POLRA, Anti-RpIILS, Anti-RPBh1, Anti-RPOL2, Anti-hRPB220, Anti-RPO2
eCl@ss:
32160702
NACRES:
NA.41

Nível de qualidade

100

fonte biológica

mouse

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

CTD4H8, monoclonal

species reactivity

mouse, Saccharomyces cerevisiae, rat, human

embalagem

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

Isotipo

IgG1

nº de adesão NCBI

nº de adesão UniProt

enviado em

dry ice

Gene Information

human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)

Descrição geral

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Especificidade

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

Imunogênio

Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

Aplicação

Research Sub Category
Transcription Factors
Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Research Category
Epigenetics & Nuclear Function

Qualidade

Routinely evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts

Descrição-alvo

210-220 kDa

forma física

Format: Purified
Protein G Chromatography
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análise

Control
A431 nuclear extract.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the {beta}-globin gene locus.
Zhou Z, Li X, Deng C, Ney PA, Huang S, Bungert J
The Journal of Biological Chemistry null
Sp1 and Sp3 foci distribution throughout mitosis.
He, Shihua and Davie, James R
Journal of Cell Science, 119, 1063-1070 (2006)
Coordination of replication and transcription along a Drosophila chromosome.
MacAlpine, DM; Rodriguez, HK; Bell, SP
Genes & Development null
Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast.
Huang, YC; Chen, HT; Teng, SC
RNA null
Inhibition of activator protein-1 by sulforaphane involves interaction with cysteine in the cFos DNA-binding domain: implications for chemoprevention of UVB-induced skin cancer.
Dickinson, SE; Melton, TF; Olson, ER; Zhang, J; Saboda, K; Bowden, GT
Cancer Research null

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